An adverse prenatal environment can have long-term effects on the health of the child, which can manifest much later in life and lead, for example, to the development of respiratory diseases. The molecular mechanisms transmitting early exposure effects to the phenotypic scale are incompletely understood, and appropriate in vitro systems are missing. We are particularly interested in persistent epigenetic modifications and their functional translation in response to such early exposures that might help to understand the development of respiratory complications. This Ph.D. project will complement our epidemiological studies by establishing and applying in vitro models for different early adverse exposure scenarios.
Charité-BIH Centrum für Therapieforschung
The job holder will be part of a research group focusing on epigenetic modifications and health effects. In his/her project, the job holder will establish in vitro models to investigate the consequences of chemical/particulate matter exposure. He/she will establish lung organoid cultures to study the priming effects of an adverse environment during development. Beyond organoid cell culture, different molecular biology and imaging techniques will be applied, including but not limited to e.g., pyrosequencing and qPCR. Single-cell RNA sequencing studies will complement epigenetic studies.
master degree in biology, biochemistry, molecular biotechnology or related disciplines
experiences with in vitro experimental studies and human cell culture models
fluent in spoken and written English
collaborative skills, enthusiasm, willingness to above-average commitment